In vitro proliferation for shoot tip and nodal explants of rosa canina

Abstract

Most previous studies of in vitro rose cultures used medium supplemented with a combination of 6-benzylaminopurine (BAP) and Naphthalene acetic acid (NAA), but the medium with the combination of BAP and Gibberellic acid (GA₃) needs to be studied.  The present study aims to test the effect of the BAP and GA₃ combination compared to the BAP and NAA combination on Rosa canina shoot multiplication. Shoot tip and nodal explants were cultured on MS media containing two concentrations of BAP (0.5 and 0.75 mg/L) combined with two levels (0.25 and 0.5 mg/L) of either NAA or GA₃.  Medium content combination of BAP combined with GA₃ showed maximum in vitro shoot induction and multiplication for both shoot tip and nodal explants compared with the medium content combination of BAP with NAA. The medium content combination of 0.75 mg/L BAP with 0.5 mg/L GA₃ showed a maximum number of shoots, leaves number, and shoot length for both shoot tip and nodal explant of rose during shoot induction and multiplication period.